Journal: F1000Research

Article Title: An unexpected effect of TNF-α on F508del-CFTR maturation and function

doi: 10.12688/f1000research.6683.2

Figure Lengend Snippet: Differentiated primary HBE cell cultures grown at an air-liquid interface were incubated with 50ng/ml TNFα for 10–30min, 3–6h and 24h. CFTR immunodetection was performed with 24.1 anti-CFTR antibody and analyzed with confocal microscopy. Green staining represents CFTR (Alexa Fluor 488), red color staining represents ZO-1 protein of tight junctions (Alexa Fluor 594) and blue DAPI staining visualizes nuclei. Independent TNFα treatments and CFTR immunodetection were performed on HBE cells from three different F508del/F508del CF patients. Representative images of one experiment are demonstrated (Scale bars = 20µm).

Article Snippet: Anti-CFTR antibodies (abs): MM13-4 mouse monoclonal ab against N-terminus of CFTR, (Millipore, France, 05-581); 24-1 mouse monoclonal ab against C-terminus of CFTR (R&D Systems, MAB, 25031).

Techniques: Incubation, Immunodetection, Confocal Microscopy, Staining

Journal: iScience

Article Title: The endoplasmic reticulum membrane complex promotes proteostasis of GABA A receptors

doi: 10.1016/j.isci.2022.104754

Figure Lengend Snippet:

Article Snippet: The rabbit polyclonal anti-GABA A α1 antibody came from R&D systems (catalog #: PPS022).

Techniques: Virus, Recombinant, Modification, Saline, Transfection, Magnetic Beads, Protease Inhibitor, Mutagenesis, Titration, Control, Plasmid Preparation, Software